Quality Control

Cyanobacterial LEGE strains are normally kept at 10–30 μmol photons m−2 s−1 under 12h/12h or 14h/10h light/dark cycles. The range of controlled temperature conditions at LEGE are 14ºC, 19ºC (for most strains), and 25ºC

Culture Conditions and Quality Assurance

The LEGE culture collection is ISO 9001:2015 certified, reflecting our commitment to high-quality standards in strain preservation, documentation, and customer service.

LEGE cyanobacterial strains are cultured under controlled conditions, typically at 10–30 μmol photons m⁻² s⁻¹ with 12h/12h or 14h/10h light/dark cycles. Temperature settings include 14ºC, 19ºC (standard for most strains), and 25ºC. Each strain’s specific cultivation conditions can be found on its individual page on our Database.

Strains are maintained through regular subculturing in six standardized media (see Knowledgebase), with intervals ranging from 2 to 6 months depending on strain sensitivity. In addition to active cultures, some strains are cryopreserved at –80ºC. A complete cryopreserved collection is being developed at –150ºC to ensure long-term genetic stability and reduce contamination risk

Prior to any shipment, all strains undergo visual inspection to confirm morphological integrity, growth, and fitness. Toxin-producing strains are routinely analyzed using advanced analytical chemistry at CIIMAR facilities.

Citation and Acknowledgment

Users must cite the LEGE strain number(s) in all publications and patent applications and acknowledge the LEGE culture collection as the source. We kindly request notification of any publications involving LEGE strains.

 

 

 

HANDLING AND SAFETY ISSUES
Tubes/cultures sent by LEGE should only be opened and used by trained persons, in suitable laboratory conditions.
The volume and concentration of culture supplied is unlikely to be a serious hazard to laboratory workers, the community, livestock, or the environment.
LEGE is not responsible to any damage or injury that might happen after arrival.
The customer should maintain, grow, use or dispose the cultures in accordance with appropriate procedures and precautions to avoid risks to persons and to the environment.
Some LEGE strains are known to produce common cyanotoxins. In such cases, customers will be warned about it. Specific hazards that may be encountered when processing toxic cyanobacteria and recommended control measures can be found in Stewart et al. (2009).
LEGE strains may produce other secondary metabolites with unknown toxicity, all strains should be treated with caution.

In all situations, customers are advised to use a Class II biosafety cabinet for work with the cultures, and an autoclave or other suitable method (see Stewart et al., 2009) for disposal and decontamination within the lab.

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