Antibacterial and antibiofilm activity of LEGE 00249 lipids

New paper about the antibacterial and antibiofilm activity of Sphaerospermopsis sp. LEGE 00249 lipids. This strain was selected after screening 600 microalgae and cyanobacteria strains from two culture collections (one of them LEGE-CC).

Cepas V, Gutiérrez-Del-Río I, López Y, Redondo-Blanco S, Gabasa Y, Iglesias MJ, Soengas R, Fernández-Lorenzo A, López-Ibáñez S, Villar CJ, Martins CB, Ferreira JD, Assunção MFG, Santos LMA, Morais J, Castelo-Branco R, Reis MA, Vasconcelos V, López-Ortiz F, Lombó F, Soto SM. Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity. Marine Drugs. 2021; 19(12):675. – Link to Paper


Lipids are one of the primary metabolites of microalgae and cyanobacteria, which enrich their utility in the pharmaceutical, feed, cosmetic, and chemistry sectors. This work describes the isolation, structural elucidation, and the antibiotic and antibiofilm activities of diverse lipids produced by different microalgae and cyanobacteria strains from two European collections (ACOI and LEGE-CC). Three microalgae strains and one cyanobacteria strain were selected for their antibacterial and/or antibiofilm activity after the screening of about 600 strains carried out under the NoMorFilm European project. The total organic extracts were firstly fractionated using solid phase extraction methods, and the minimum inhibitory concentration and minimal biofilm inhibitory concentration against an array of human pathogens were determined. The isolation was carried out by bioassay-guided HPLC-DAD purification, and the structure of the isolated molecules responsible for the observed activities was determined by HPLC-HRESIMS and NMR methods. Sulfoquinovosyldiacylglycerol, monogalactosylmonoacylglycerol, sulfoquinovosylmonoacylglycerol, α-linolenic acid, hexadeca-4,7,10,13-tetraenoic acid (HDTA), palmitoleic acid, and lysophosphatidylcholine were found among the different active sub-fractions selected. In conclusion, cyanobacteria and microalgae produce a great variety of lipids with antibiotic and antibiofilm activity against the most important pathogens causing severe infections in humans. The use of these lipids in clinical treatments alone or in combination with antibiotics may provide an alternative to the current treatments.